[HTML][HTML] Temperature-modulated diversity of TRPV4 channel gating: activation by physical stresses and phorbol ester derivatives through protein kinase C-dependent …
X Gao, L Wu, RG O'Neil - Journal of Biological Chemistry, 2003 - ASBMB
The TRPV4 calcium-permeable channel was cloned from mouse kidney M-1 cells, and the
effect of temperature modulation on channel gating/activation by physical and chemical
signals was evaluated. A TRPV4 cDNA construct with a C-terminal V5 epitope was stably
transfected into human embryonic kidney (HEK) 293 and Chinese hamster ovary cells
resulting in high levels of expression at the plasma membrane. Channel activation was
assessed from changes in calcium influx (fura-2 fluorescence measurements) or whole cell …
effect of temperature modulation on channel gating/activation by physical and chemical
signals was evaluated. A TRPV4 cDNA construct with a C-terminal V5 epitope was stably
transfected into human embryonic kidney (HEK) 293 and Chinese hamster ovary cells
resulting in high levels of expression at the plasma membrane. Channel activation was
assessed from changes in calcium influx (fura-2 fluorescence measurements) or whole cell …
