[CITATION][C] O-Linked Oligosaccharide on IgA1 Hinge Region in IgA Nephropathy1
Y Hiki, A Horii, H Iwase, A Tanaka, Y Toda, K Hotta… - IgA …, 1995 - karger.com
Y Hiki, A Horii, H Iwase, A Tanaka, Y Toda, K Hotta, Y Kobayashi
IgA Nephropathy, 1995•karger.comMaterials and Methods Sera and Separation of IgA 1 Thirty-one sera (IgAN, n= 12; other
primary GN, n= 11; healthy controls, n= 8) were subjects of this study. IgA 1 from 3ml of each
serum sample was isolated by Jacalin agarose affinity chromatography [4]. Monoclonal IgA
1 separated by ion-exchange chromatography was purchased from Chemicon International
Inc.(El Segundo, Calif., USA). Structure of IgA 1 Fragment Containing O-Glycans Two IgA 1
samples originating from the different sources were subjects for the analysis. One was …
primary GN, n= 11; healthy controls, n= 8) were subjects of this study. IgA 1 from 3ml of each
serum sample was isolated by Jacalin agarose affinity chromatography [4]. Monoclonal IgA
1 separated by ion-exchange chromatography was purchased from Chemicon International
Inc.(El Segundo, Calif., USA). Structure of IgA 1 Fragment Containing O-Glycans Two IgA 1
samples originating from the different sources were subjects for the analysis. One was …
Materials and Methods
Sera and Separation of IgA 1 Thirty-one sera (IgAN, n= 12; other primary GN, n= 11; healthy controls, n= 8) were subjects of this study. IgA 1 from 3ml of each serum sample was isolated by Jacalin agarose affinity chromatography [4]. Monoclonal IgA 1 separated by ion-exchange chromatography was purchased from Chemicon International Inc.(El Segundo, Calif., USA).
Structure of IgA 1 Fragment Containing O-Glycans Two IgA 1 samples originating from the different sources were subjects for the analysis. One was separated from a healthy individual by Jacalin affinity chromatography, and another was a monoclonal IgA 1 prepared by ion-exchange chromatography. Each of the IgA 1 molecules was pyridylethylated to cleave the disulfide bonds (PE-IgA 1), and then treated with trypsin. The fragments of PE-IgA 1 were fractionated using reverse-phase column (Asahipak HIKARISIL-CI8, 4.6 x 250 mm). Figure 3 shows a sample of the peak pattern from this analysis.
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