γ-Interferon stimulates production of 1, 25-dihydroxyvitamin D3 by normal human macrophages
HP Koeffler, H Reichel, JE Bishop… - … and biophysical research …, 1985 - Elsevier
HP Koeffler, H Reichel, JE Bishop, AW Norman
Biochemical and biophysical research communications, 1985•ElsevierWe show for the first time that normal human pulmonary alveolar macrophages (PAM)
markedly enhance their basal rate of the production of [3 H]--1, 25 (OH) 2 D 3 when cultured
in the presence of recombinant γ-interferon (γ-IFN). The rate of conversion of [3 H]-25 (OH) D
3 to [3 H]-1, 25 (OH) 2 D 3 was dose-dependent in a linear fashion. A maximal production of
1, 25 (OH) 2 D 3 by PAM occured after exposure of PAM to γ-IFN for one day. This maximum
plateau-level was sustained for at least five days. The authenticity of the putative 1, 25 (OH) …
markedly enhance their basal rate of the production of [3 H]--1, 25 (OH) 2 D 3 when cultured
in the presence of recombinant γ-interferon (γ-IFN). The rate of conversion of [3 H]-25 (OH) D
3 to [3 H]-1, 25 (OH) 2 D 3 was dose-dependent in a linear fashion. A maximal production of
1, 25 (OH) 2 D 3 by PAM occured after exposure of PAM to γ-IFN for one day. This maximum
plateau-level was sustained for at least five days. The authenticity of the putative 1, 25 (OH) …
We show for the first time that normal human pulmonary alveolar macrophages (PAM) markedly enhance their basal rate of the production of [3H]--1,25(OH)2D3 when cultured in the presence of recombinant γ-interferon (γ-IFN). The rate of conversion of [3H]-25(OH)D3 to [3H]-1,25(OH)2D3 was dose-dependent in a linear fashion. A maximal production of 1,25(OH)2D3 by PAM occured after exposure of PAM to γ-IFN for one day. This maximum plateau-level was sustained for at least five days. The authenticity of the putative 1,25(OH)2D3 obtained from PAM was tested by demonstrating the exact comigration of [3H]-1,25(OH)2D3 with chemically synthesized 1,25(OH)2D3 in four different HPLC-systems.
Elsevier