Phosphorylated insulin-like growth factor (IGF)-binding protein-1 (IGFBP-1) inhibits while non-phosphorylated IGFBP-1 stimulates IGF-I-induced amino acid uptake by …
J Yu, M Iwashita, Y Kudo, Y Takeda - Growth Hormone & IGF Research, 1998 - Elsevier
The effects of phosphorylated insulin-like growth factor-binding protein (plGFBP-1) and non-
phosphorylated (nplGFBP-1) IGFBP-1 on amino acid uptake induced by IGF-I were studied
using cultured trophoblast cells. Trophoblast cells obtained from term pregnancy were
incubated with indicated concentrations of plGFBP-1 or nplGFBP-1 for 24 h and further
incubated with 10 nM IGF-I for 3 h. Cells were then incubated with 3H-α-amino isobutyric
acid (3H-AIB) for 30 min. Both plGFBP-1 and nplGFBP-1 alone had no effect on 3H-AIB …
phosphorylated (nplGFBP-1) IGFBP-1 on amino acid uptake induced by IGF-I were studied
using cultured trophoblast cells. Trophoblast cells obtained from term pregnancy were
incubated with indicated concentrations of plGFBP-1 or nplGFBP-1 for 24 h and further
incubated with 10 nM IGF-I for 3 h. Cells were then incubated with 3H-α-amino isobutyric
acid (3H-AIB) for 30 min. Both plGFBP-1 and nplGFBP-1 alone had no effect on 3H-AIB …
