[HTML][HTML] Mechanism and cellular applications of a green fluorescent protein-based halide sensor
S Jayaraman, P Haggie, RM Wachter… - Journal of Biological …, 2000 - ASBMB
We report the application of a targetable green fluorescent protein-based cellular halide
indicator. Fluorescence titrations of the purified recombinant yellow fluorescent protein YFP-
H148Q indicated ap K a of 7.14 in the absence of Cl−, which increased to 7.86 at 150
mmCl−. At pH 7.5, YFP-H148Q fluorescence decreased maximally by∼ 2-fold with a KD of
100 mmCl−. YFP-H148Q had a fluorescence lifetime of 3.1 ns that was independent of pH
and [Cl−]. Circular dichroism and absorption spectroscopy revealed distinct Cl−-dependent …
indicator. Fluorescence titrations of the purified recombinant yellow fluorescent protein YFP-
H148Q indicated ap K a of 7.14 in the absence of Cl−, which increased to 7.86 at 150
mmCl−. At pH 7.5, YFP-H148Q fluorescence decreased maximally by∼ 2-fold with a KD of
100 mmCl−. YFP-H148Q had a fluorescence lifetime of 3.1 ns that was independent of pH
and [Cl−]. Circular dichroism and absorption spectroscopy revealed distinct Cl−-dependent …
