Objective: To examine the distribution and relative levels of vascular endothelial growth
factor (VEGF) in the nondiabetic and preproliferative diabetic human retina and choroid.
Methods: Immunohistochemical localization was performed on frozen sections from
cryopreserved postmortem human tissue using a polyclonal antibody against VEGF and a
streptavidin peroxidase system. Eyes from 5 subjects without diabetes and 8 subjects with
diabetes were examined and analyzed using a 7-point immunohistochemical grading …