Inhibition of NADH-linked oxidation in brain mitochondria by 1-methyl-4-phenyl-pyridine, a metabolite of the neurotoxin, 1-methyl-4-phenyl-1, 2, 5, 6-tetrahydropyridine
WJ Nicklas, I Vyas, RE Heikkila - Life sciences, 1985 - Elsevier
WJ Nicklas, I Vyas, RE Heikkila
Life sciences, 1985•Elseviermethyl-4-phenylpyridine (MPP+), a major metabolite of the neurotoxin, 1-methyl-4-phenyl-1,
2, 5, 6-tetrahydropyridine (MPTP) inhibited the ADP-stimulated and uncoupled oxidation of
NADH-linked substrates by brain mitochondrial preparations. MPTP itself was ineffective.
The apparent K i's for MPP+ inhibition of pyruvate or glutamate oxidation by purified rat brain
mitochondria were approximately 300 and 400 μM, respectively; with mouse brain
mitochondria the values were lower, 60 and 150 μ M, respectively. Succinate oxidation was …
2, 5, 6-tetrahydropyridine (MPTP) inhibited the ADP-stimulated and uncoupled oxidation of
NADH-linked substrates by brain mitochondrial preparations. MPTP itself was ineffective.
The apparent K i's for MPP+ inhibition of pyruvate or glutamate oxidation by purified rat brain
mitochondria were approximately 300 and 400 μM, respectively; with mouse brain
mitochondria the values were lower, 60 and 150 μ M, respectively. Succinate oxidation was …
Abstract
1-methyl-4-phenylpyridine (MPP+), a major metabolite of the neurotoxin, 1-methyl-4-phenyl-1,2,5,6-tetrahydropyridine (MPTP) inhibited the ADP-stimulated and uncoupled oxidation of NADH-linked substrates by brain mitochondrial preparations. MPTP itself was ineffective. The apparent Ki's for MPP+ inhibition of pyruvate or glutamate oxidation by purified rat brain mitochondria were approximately 300 and 400 μM, respectively; with mouse brain mitochondria the values were lower, 60 and 150 μ M, respectively. Succinate oxidation was unaffected by either compound. Compromise of mitochondrial oxidate capacity by MPP+ could be an important factor in mechanisms underlying the toxicity of MPTP.
Elsevier