[HTML][HTML] Expression of the osteoblast differentiation factor RUNX2 (Cbfa1/AML3/Pebp2αA) is inhibited by tumor necrosis factor-α
L Gilbert, X He, P Farmer, J Rubin, H Drissi… - Journal of Biological …, 2002 - ASBMB
The transcription factor RUNX2 (Cbfa1/AML3/Pebp2αA) is a critical regulator of osteoblast
differentiation. We investigated the effect of the inflammatory cytokine tumor necrosis factor α
(TNF) on the expression of RUNX2 because TNF is known to inhibit differentiation of
osteoblasts from pluripotent progenitor cells. TNF treatment of fetal calvaria precursor cells
or MC3T3-E1 clonal pre-osteoblastic cells caused a dose-dependent suppression of
RUNX2 steady state mRNA as measured by reverse transcription-PCR. The IC 50 for TNF …
differentiation. We investigated the effect of the inflammatory cytokine tumor necrosis factor α
(TNF) on the expression of RUNX2 because TNF is known to inhibit differentiation of
osteoblasts from pluripotent progenitor cells. TNF treatment of fetal calvaria precursor cells
or MC3T3-E1 clonal pre-osteoblastic cells caused a dose-dependent suppression of
RUNX2 steady state mRNA as measured by reverse transcription-PCR. The IC 50 for TNF …