Publisher Summary Glucose-6-phosphate dehydrogenase catalyzes the oxidation of
glucose 6-phosphate in the presence of nicotinamide adenine dinucleotide phosphate
kinase (NADP+) and is the first enzyme in the pentose shunt pathway. This enzyme from
many sources is successfully purified by general ligand affinity chromatography using the
derivatives of NADP+ as immobilized ligands. This chapter describes the assay method,
purification procedure, and properties of glucose-6-phosphate dehydrogenase. The activity …