RNAi-based analysis of CAP, Cbl, and CrkII function in the regulation of GLUT4 by insulin
P Mitra, X Zheng, MP Czech - Journal of Biological Chemistry, 2004 - ASBMB
Stimulation of glucose transport by insulin in cultured adipocytes through translocation of
intracellular GLUT4 glucose transporters to the plasma membrane has been suggested to
require phosphatidylinositol (PI) 3-kinase-dependent and independent mechanisms. To test
the involvement of a PI 3-kinase-independent pathway leading to activation of the TC10
GTPase, the putative intermediates CAP, c-Cbl, Cbl-b, and CrkII were selectively depleted in
3T3-L1 adipocytes using highly efficient small interfering (si) RNAs. Simultaneous depletion …
intracellular GLUT4 glucose transporters to the plasma membrane has been suggested to
require phosphatidylinositol (PI) 3-kinase-dependent and independent mechanisms. To test
the involvement of a PI 3-kinase-independent pathway leading to activation of the TC10
GTPase, the putative intermediates CAP, c-Cbl, Cbl-b, and CrkII were selectively depleted in
3T3-L1 adipocytes using highly efficient small interfering (si) RNAs. Simultaneous depletion …
