[HTML][HTML] Neuronal Ca2+ sensor-1/frequenin functions in an autocrine pathway regulating Ca2+ channels in bovine adrenal chromaffin cells
JL Weiss, DA Archer, RD Burgoyne - Journal of Biological Chemistry, 2000 - ASBMB
NCS-1/frequenin belongs to a family of EF-hand-containing Ca 2+ sensors expressed
mainly in neurons. Overexpression of NCS-1/frequenin has been shown to stimulate
neurotransmitter release but little else is known of its cellular roles. We have constructed an
EF-hand mutant, NCS-1 (E120Q), as a likely dominant inhibitor of cellular NCS-1 function.
Recombinant NCS-1 (E120Q) showed an impaired Ca 2+-dependent conformational
change but could still bind to cellular proteins. Transient expression of this mutant, but not …
mainly in neurons. Overexpression of NCS-1/frequenin has been shown to stimulate
neurotransmitter release but little else is known of its cellular roles. We have constructed an
EF-hand mutant, NCS-1 (E120Q), as a likely dominant inhibitor of cellular NCS-1 function.
Recombinant NCS-1 (E120Q) showed an impaired Ca 2+-dependent conformational
change but could still bind to cellular proteins. Transient expression of this mutant, but not …
