Purification of myristoylated and nonmyristoylated neuronal calcium sensor-1 using single-step hydrophobic interaction chromatography
JR Fisher, Y Sharma, S Iuliano, RA Piccioti… - Protein Expression and …, 2000 - Elsevier
Neuronal calcium sensors (NCSs) belong to a family of Ca2+-binding proteins, which serve
important functions in neurotransmission, and are highly conserved from yeast to humans.
Overexpression of the neuronal calcium sensor-1, called frequenin in the fruit fly and in frog,
increases the release of neurotransmitters. Studying the functional role of frequenin in
mammals and understanding its structural dynamics is critically dependent on the
availability of active purified protein. Neuronal calcium sensors like other members of the …
important functions in neurotransmission, and are highly conserved from yeast to humans.
Overexpression of the neuronal calcium sensor-1, called frequenin in the fruit fly and in frog,
increases the release of neurotransmitters. Studying the functional role of frequenin in
mammals and understanding its structural dynamics is critically dependent on the
availability of active purified protein. Neuronal calcium sensors like other members of the …
