In vitro studies suggest that the mitochondrialglycerol-3-phosphate acyltransferase-1
(mtGPAT1) isoform catalyzes the initialand rate-controlling step in glycerolipid synthesis and
aids in partitioningacyl-CoAs toward triacylglycerol synthesis and away from degradative
pathways. To determine whether the absence of mtGPAT1 would increase oxidation ofacyl-
CoAs and restrict the development of hepatic steatosis, we fed wild typeand mtGPAT1–/–
mice a diet high in fat and sucrose (HH) for 4 months to induce the development of obesity …