Identification of a functional phosphatidylinositol 3, 4, 5-trisphosphate binding site in the epithelial Na+ channel
O Pochynyuk, A Staruschenko, Q Tong… - Journal of Biological …, 2005 - ASBMB
Membrane phospholipids, such as phosphatidylinositol 3, 4, 5-trisphosphate (PI (3, 4, 5) P
3), are signaling molecules that can directly modulate the activity of ion channels, including
the epithelial Na+ channel (ENaC). Whereas PI (3, 4, 5) P 3 directly activates ENaC, its
binding site within the channel has not been identified. We identify here a region of γ-
mENaC just following the second trans-membrane domain (residues 569–583) important to
PI (3, 4, 5) P 3 binding and regulation. Deletion of this track decreases activity of ENaC …
3), are signaling molecules that can directly modulate the activity of ion channels, including
the epithelial Na+ channel (ENaC). Whereas PI (3, 4, 5) P 3 directly activates ENaC, its
binding site within the channel has not been identified. We identify here a region of γ-
mENaC just following the second trans-membrane domain (residues 569–583) important to
PI (3, 4, 5) P 3 binding and regulation. Deletion of this track decreases activity of ENaC …
