Identification of mitochondrial branched chain aminotransferase and its isoforms in rat tissues.
SM Hutson, R Wallin, TR Hall - Journal of Biological Chemistry, 1992 - ASBMB
The tissue distribution and subcellular location of branched chain aminotransferase was
analyzed using polyclonal antibodies against the enzyme purified from rat heart
mitochondria (BCATm). Immunoreactive proteins were visualized by immunoblotting. The
antiserum recognized a 41-kDa protein in the 100,000 xg supernatant from a rat heart
mitochondrial sonicate. The 41-kDa protein was always present in mitochondria which
contained branched chain aminotransferase activity, skeletal muscle, kidney, stomach, and …
analyzed using polyclonal antibodies against the enzyme purified from rat heart
mitochondria (BCATm). Immunoreactive proteins were visualized by immunoblotting. The
antiserum recognized a 41-kDa protein in the 100,000 xg supernatant from a rat heart
mitochondrial sonicate. The 41-kDa protein was always present in mitochondria which
contained branched chain aminotransferase activity, skeletal muscle, kidney, stomach, and …