[CITATION][C] [42] Branched-chain α-keto acid dehydrogenase phosphatase and its inhibitor protein from bovine kidney
Z Damuni, LJ Reed - Methods in Enzymology, 1988 - Elsevier
Reagents. Dilution buffer: BCKDH phosphatase is diluted in 50 mM imidazole chloride
(Sigma Chemical Co., St. Louis, MO, grade III), pH 7.3, 10% glycerol, 0.1 mM
ethylenediaminetetraacetic acid (EDTA), 0.1 mM ethylene glycol bis (fl-aminoethyl ether)-N,
N'-tetraacetic acid (EGTA), 0.1 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM
benzamidine, 1 mM dithiothreitol (buffer B) containing bovine serum albumin (I mg/ml).
(Sigma Chemical Co., St. Louis, MO, grade III), pH 7.3, 10% glycerol, 0.1 mM
ethylenediaminetetraacetic acid (EDTA), 0.1 mM ethylene glycol bis (fl-aminoethyl ether)-N,
N'-tetraacetic acid (EGTA), 0.1 mM phenylmethylsulfonyl fluoride (PMSF), 1 mM
benzamidine, 1 mM dithiothreitol (buffer B) containing bovine serum albumin (I mg/ml).