Firefly Luciferase–Based Dynamic Bioluminescence Imaging: A Noninvasive Technique to Assess Tumor Angiogenesis
A Sun, L Hou, T Prugpichailers, J Dunkel… - …, 2010 - journals.lww.com
OBJECTIVE Bioluminescence imaging (BLI) is emerging as a cost-effective, high-
throughput, noninvasive, and sensitive imaging modality to monitor cell growth and
trafficking. We describe the use of dynamic BLI as a noninvasive method of assessing vessel
permeability during brain tumor growth. METHODS With the use of stereotactic technique,
10 5 firefly luciferase–transfected GL26 mouse glioblastoma multiforme cells were injected
into the brains of C57BL/6 mice (n= 80). After intraperitoneal injection of D-luciferin (150 …
throughput, noninvasive, and sensitive imaging modality to monitor cell growth and
trafficking. We describe the use of dynamic BLI as a noninvasive method of assessing vessel
permeability during brain tumor growth. METHODS With the use of stereotactic technique,
10 5 firefly luciferase–transfected GL26 mouse glioblastoma multiforme cells were injected
into the brains of C57BL/6 mice (n= 80). After intraperitoneal injection of D-luciferin (150 …
