Quantitative tracking of T cell clones after haematopoietic stem cell transplantation
IZ Mamedov, OV Britanova, DA Bolotin… - EMBO molecular …, 2011 - embopress.org
IZ Mamedov, OV Britanova, DA Bolotin, AV Chkalina, DB Staroverov, IV Zvyagin…
EMBO molecular medicine, 2011•embopress.orgAutologous haematopoietic stem cell transplantation is highly efficient for the treatment of
systemic autoimmune diseases, but its consequences for the immune system remain poorly
understood. Here, we describe an optimized RNA‐based technology for unbiased
amplification of T cell receptor beta‐chain libraries and use it to perform the first detailed,
quantitative tracking of T cell clones during 10 months after transplantation. We show that
multiple clones survive the procedure, contribute to the immune response to activated …
systemic autoimmune diseases, but its consequences for the immune system remain poorly
understood. Here, we describe an optimized RNA‐based technology for unbiased
amplification of T cell receptor beta‐chain libraries and use it to perform the first detailed,
quantitative tracking of T cell clones during 10 months after transplantation. We show that
multiple clones survive the procedure, contribute to the immune response to activated …
Abstract
Autologous haematopoietic stem cell transplantation is highly efficient for the treatment of systemic autoimmune diseases, but its consequences for the immune system remain poorly understood. Here, we describe an optimized RNA‐based technology for unbiased amplification of T cell receptor beta‐chain libraries and use it to perform the first detailed, quantitative tracking of T cell clones during 10 months after transplantation. We show that multiple clones survive the procedure, contribute to the immune response to activated infections, and form a new skewed and stable T cell receptor repertoire.
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