[HTML][HTML] Distinct roles in autophagy and importance in infectivity of the two ATG4 cysteine peptidases of Leishmania major
RAM Williams, JC Mottram, GH Coombs - Journal of Biological Chemistry, 2013 - ASBMB
Macroautophagy in Leishmania, which is important for the cellular remodeling required
during differentiation, relies upon the hydrolytic activity of two ATG4 cysteine peptidases
(ATG4. 1 and ATG4. 2). We have investigated the individual contributions of each ATG4 to
Leishmania major by generating individual gene deletion mutants (Δatg4. 1 and Δatg4. 2);
double mutants could not be generated, indicating that ATG4 activity is required for parasite
viability. Both mutants were viable as promastigotes and infected macrophages in vitro and …
during differentiation, relies upon the hydrolytic activity of two ATG4 cysteine peptidases
(ATG4. 1 and ATG4. 2). We have investigated the individual contributions of each ATG4 to
Leishmania major by generating individual gene deletion mutants (Δatg4. 1 and Δatg4. 2);
double mutants could not be generated, indicating that ATG4 activity is required for parasite
viability. Both mutants were viable as promastigotes and infected macrophages in vitro and …