Isolation of cardiac myocytes and fibroblasts from neonatal rat pups
HB Golden, D Gollapudi, F Gerilechaogetu, J Li… - … : methods and protocols, 2012 - Springer
Cardiovascular development: methods and protocols, 2012•Springer
Neonatal rat ventricular myocytes (NRVM) and fibroblasts (FBs) serve as in vitro models for
studying fundamental mechanisms underlying cardiac pathologies, as well as identifying
potential therapeutic targets. Both cell types are relatively easy to culture as monolayers and
can be manipulated using molecular and pharmacological tools. Because NRVM cease to
proliferate after birth, and FBs undergo phenotypic changes and senescence after a few
passages in tissue culture, primary cultures of both cell types are required for experiments …
studying fundamental mechanisms underlying cardiac pathologies, as well as identifying
potential therapeutic targets. Both cell types are relatively easy to culture as monolayers and
can be manipulated using molecular and pharmacological tools. Because NRVM cease to
proliferate after birth, and FBs undergo phenotypic changes and senescence after a few
passages in tissue culture, primary cultures of both cell types are required for experiments …
Abstract
Neonatal rat ventricular myocytes (NRVM) and fibroblasts (FBs) serve as in vitro models for studying fundamental mechanisms underlying cardiac pathologies, as well as identifying potential therapeutic targets. Both cell types are relatively easy to culture as monolayers and can be manipulated using molecular and pharmacological tools. Because NRVM cease to proliferate after birth, and FBs undergo phenotypic changes and senescence after a few passages in tissue culture, primary cultures of both cell types are required for experiments. Below we describe methods that provide good cell yield and viability of primary cultures of NRVM and FBs from 0 to 3-day-old neonatal rat pups.
Springer
