Metabolic assays for detection of neutral fat stores
JM Baumann, L Kokabee, X Wang, Y Sun, J Wong… - Bio …, 2015 - bio-protocol.org
Lipid droplets (LDs) are ubiquitous intracellular structures whose formation, growth, and
maintenance are highly regulated (Wang et al., 2013; Ranall et al., 2011; Goodman, 2009).
Lipid metabolism and droplet dynamics are of considerable interest to agriculture, biofuel
production, viral pathology, nutrition, and cancer biology (Walther and Farese, 2009; Liu et
al., 2010). Accumulation of fatty acids and neutral lipids in nonadipose tissues is cytotoxic
(Kourtidis et al., 2009). BODIPY 493/503 (4, 4-Difluoro-1, 3, 5, 7, 8-Pentamethyl-4-Bora-3a …
maintenance are highly regulated (Wang et al., 2013; Ranall et al., 2011; Goodman, 2009).
Lipid metabolism and droplet dynamics are of considerable interest to agriculture, biofuel
production, viral pathology, nutrition, and cancer biology (Walther and Farese, 2009; Liu et
al., 2010). Accumulation of fatty acids and neutral lipids in nonadipose tissues is cytotoxic
(Kourtidis et al., 2009). BODIPY 493/503 (4, 4-Difluoro-1, 3, 5, 7, 8-Pentamethyl-4-Bora-3a …
