Site-directed mutants of human RECQ1 reveal functional importance of the zinc binding domain
RecQ helicases are a highly conserved family of ATP-dependent DNA-unwinding enzymes
with key roles in DNA replication and repair in all kingdoms of life. The RECQ1 gene
encodes the most abundant RecQ homolog in humans. We engineered full-length RECQ1
harboring point mutations in the zinc-binding motif (amino acids 419–480) within the
conserved RecQ-specific-C-terminal (RQC) domain known to be critical for diverse
biochemical and cellular functions of RecQ helicases. Wild-type RECQ1 contains a zinc ion …
with key roles in DNA replication and repair in all kingdoms of life. The RECQ1 gene
encodes the most abundant RecQ homolog in humans. We engineered full-length RECQ1
harboring point mutations in the zinc-binding motif (amino acids 419–480) within the
conserved RecQ-specific-C-terminal (RQC) domain known to be critical for diverse
biochemical and cellular functions of RecQ helicases. Wild-type RECQ1 contains a zinc ion …
