[HTML][HTML] Structural characterization of the human eukaryotic initiation factor 3 protein complex by mass spectrometry
Protein synthesis in mammalian cells requires initiation factor eIF3, an∼ 800-kDa protein
complex that plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40
S ribosomal subunit to form the 48 S initiation complex. The eIF3 complex also prevents
premature association of the 40 and 60 S ribosomal subunits and interacts with other
initiation factors involved in start codon selection. The molecular mechanisms by which eIF3
exerts these functions are poorly understood. Since its initial characterization in the 1970s …
complex that plays a central role in binding of initiator methionyl-tRNA and mRNA to the 40
S ribosomal subunit to form the 48 S initiation complex. The eIF3 complex also prevents
premature association of the 40 and 60 S ribosomal subunits and interacts with other
initiation factors involved in start codon selection. The molecular mechanisms by which eIF3
exerts these functions are poorly understood. Since its initial characterization in the 1970s …
