Abstract Setting an open-access computer assisted sperm analysis (CASA) may benefit the
evaluation of motility in mammalian sperm, especially when economic constraints do not
allow the use of a commercial system. There have been successful attempts to develop such
a device in Zebra fish sperm and the system has been used in very few studies on
mammalian spermatozoa. Against this background, the present study aimed at developing
an open-access CASA system for mammalian sperm using the horse as a model and based …