Transgenesis and gene trap methods in zebrafish by using the Tol2 transposable element

K Kawakami - Methods in cell biology, 2004 - Elsevier
Publisher Summary This chapter focuses on transgenesis and gene trap methods in
zebrafish by using the Tol2 transposable element. Zebrafish (Danio rerio) has been used as
a model animal to study vertebrate development by genetic approaches. The Tol2
transposable element, which is found in the genome of a small freshwater teleost, the
Japanese medaka fish (Oryzias latipes), belongs to the hAT family of transposons that
includes hobo of Drosophila, Ac of maize, and Tam3 of snapdragon. The zebrafish genome …